rabbit polyclonal antibody against cd4  (Proteintech)

Journal: Biomolecules

Article Title: Effect of Topical Programmed Death-Ligand1 on Corneal Epithelium in Dry Eye Mouse.

doi: 10.3390/biom14010068

Figure Lengend Snippet: Figure 3. (A) Immunofluorescence staining showing the expression of (i) PD-L1, (ii) CD4, (iii) CD3e and (iv) IL-17 localized in the corneas in the control, desiccation+PBS and desiccation+PD-L1 group. (B–E) Changes in the percentage of PD-L1, CD4, CD3e and IL-17 positive cells in the cornea. Im- munopositivity for PD-L1 is shown in higher-power fields. Immunopositivity was counted in low-power fields and calculated as relative to the total number of DAPI-positive cells. (F,G) Western blot showing the expression of CD4 and IL-17 in the cornea. (H,I) Relative expression ratio of CD4 and IL-17 to β-actin. In (B–E,H,I), data are presented with bar graph (n = 6, day 10, * p < 0.05; ** p < 0.01; *** p < 0.001 significant difference by one-way ANOVA). Original images of (F,G) can be found in Supplementary Materials.

Article Snippet: The membranes were blocked by incubation with 5% BSA in Trisbuffered saline containing Tween 20 TBST for 1 h at room temperature and incubated overnight at 4 ◦C with specific rabbit polyclonal antibodies against CD4 (1:1000, Catalog No. NBP1-19371; Novus Biologicals, Littleton, CO, USA), IL-17 (1:1000, Catalog No. ab79056; Abcam, Inc.), phosphorylated NF-kB (pNF-kB; 1:1000, Catalog No. 3033S; Cell Signaling Technology, Inc.), NF-kB (1:1000, Catalog No. 51-0500; Invitrogen, Inc.), phosphorylated IkB-α (pIkB-a 1:1000, Catalog No. 9246S; Cell Signaling Technology, Inc.), IkB-α (1:1000, Catalog No. PA5-17888; Invitrogen, Inc.), Bax protein (1:1000, Catalog No. sc-20067; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and β-actin (1:10,000, Catalog No. 5125S; Cell Signaling Technology, Inc.).

Techniques: Immunofluorescence, Staining, Expressing, Control, Western Blot

Journal: Gut Microbes

Article Title: Maternal exposure to fish oil primes offspring to harbor intestinal pathobionts associated with altered immune cell balance

doi: 10.1080/19490976.2014.997610

Figure Lengend Snippet: Rat pups born to dams fed diets rich in n-3 PUFA (fish oil) have altered colonic T cell balance. Rat dams were fed either 20% fat diets rich in either n-3 or n-6 PUFA and the offspring colons were examined for the presence of T cell markers via immunofluorescence and then quantified on colonic tissues sections. The n-3 rich diets depleted the presence of CD8+ T cells and CD4+ Foxp3+ T cells (*, P < 0.05).

Article Snippet: Paraffin-embedded colon sections cut in cross-sections (3 μm) were deparaffinized and antigen retrieval of rehydrated tissues was performed using a 1 mg/mL trypsin (Sigma) followed by incubation with the following primary antibodies: rabbit polyclonal IgG antibody raised against MPO (Thermo Scientific) to examine polymorphonuclear leukocytes; rabbit polyclonal IgG antibody raised against CD163 (Biorbyt) to examine monocytes/macrophages; fluorescein conjugated sheep IgG polyclonal antibody raised against arginase (R&D Systems) to examine M2 macrophages; rabbit monoclonal IgG antibody raised against CD8 (Cedarlane), rabbit polyclonal IgG antibody raised against CD4 (Santa Cruz Biotechnology), rabbit polyclonal IgG antibody raised against CD3 (GeneTex) and rabbit polyclonal IgG antibody raised against Foxp3 (Santa Cruz Biotechnology) were co-incubated with mouse monoclonal IgG2a antibody raised against CD4 (Abcam) to examine populations of T cells.

Techniques: Immunofluorescence

Journal: Oncology Letters

Article Title: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

doi: 10.3892/ol.2016.4439

Figure Lengend Snippet: Sequence of the primer pairs used for reverse transcription-quantitative polymerase chain reaction.

Article Snippet: The tissue slides were blocked for 10 min at room temperature (25°C), washed with phosphate-buffered saline (PBS), then incubated with the following anti-human antibodies at room temperature (25°C) for 2 h in moist dark chambers: Monoclonal mouse IgG1 against CD3 (#sc-20047), polyclonal rabbit IgG against CD4 (#sc-7219) and CD8 (#sc-7188; all from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; dilution, 1:100); monoclonal mouse IgG2a against CD45RO (#ab86080; dilution, 1:10), monoclonal rabbit IgG against CEACAM6 (#ab134074; dilution, 1:400) and monoclonal mouse IgG3 against FOXP3 (#ab450; 1:50 dilution; all from Abcam, Cambridge, MA, USA).

Techniques: Sequencing

Journal: Oncology Letters

Article Title: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

doi: 10.3892/ol.2016.4439

Figure Lengend Snippet: Representative images of CD3, CD4, CD8, CD45RO, CEACAM6 and FOXP3 staining by immunohistochemistry in normal colonic tissue, colonic adenoma and colon cancer. Images show 3,3′-diaminobenzidine tetrahydrochloride hydrate staining of positive cells. CD3, CD4, CD8 and CD45RO were immunolocalized to the membrane, CEACAM6 in the cytoplasmic and FOXP3 in the nucleus. All images were taken at ×400 scanning magnification. CD, cluster of differentiation; CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6; FOXP3, forkhead box P3.

Article Snippet: The tissue slides were blocked for 10 min at room temperature (25°C), washed with phosphate-buffered saline (PBS), then incubated with the following anti-human antibodies at room temperature (25°C) for 2 h in moist dark chambers: Monoclonal mouse IgG1 against CD3 (#sc-20047), polyclonal rabbit IgG against CD4 (#sc-7219) and CD8 (#sc-7188; all from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; dilution, 1:100); monoclonal mouse IgG2a against CD45RO (#ab86080; dilution, 1:10), monoclonal rabbit IgG against CEACAM6 (#ab134074; dilution, 1:400) and monoclonal mouse IgG3 against FOXP3 (#ab450; 1:50 dilution; all from Abcam, Cambridge, MA, USA).

Techniques: Staining, Immunohistochemistry, Membrane

Journal: Oncology Letters

Article Title: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

doi: 10.3892/ol.2016.4439

Figure Lengend Snippet: Immunohistochemical staining scores of T cell subsets in normal colonic tissue, colonic adenoma, stage I–II and stage III–IV cancer tissue. (A) CD3, (B) CD4, (C) CD8, and (D) CD45RO staining scores (n=12 for the normal group, n=38 for the adenoma group, n=40 for the stage I–II group, n=38 for the stage III–IV group). Whiskers represent the range and boxes represent the quartile values. *P<0.05 vs. normal group; ^ P<0.05 vs. adenoma group; # P<0.05 vs. stage I–II cancer group. CD, cluster of differentiation.

Article Snippet: The tissue slides were blocked for 10 min at room temperature (25°C), washed with phosphate-buffered saline (PBS), then incubated with the following anti-human antibodies at room temperature (25°C) for 2 h in moist dark chambers: Monoclonal mouse IgG1 against CD3 (#sc-20047), polyclonal rabbit IgG against CD4 (#sc-7219) and CD8 (#sc-7188; all from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; dilution, 1:100); monoclonal mouse IgG2a against CD45RO (#ab86080; dilution, 1:10), monoclonal rabbit IgG against CEACAM6 (#ab134074; dilution, 1:400) and monoclonal mouse IgG3 against FOXP3 (#ab450; 1:50 dilution; all from Abcam, Cambridge, MA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: Oncology Letters

Article Title: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

doi: 10.3892/ol.2016.4439

Figure Lengend Snippet: Expression of CD3, CD4, CD8 and CD45RO mRNA in colon tissues of each group. (A) CD3, (B) CD4, (C) CD8 and (D) CD45RO mRNA levels (n=6 for the normal group, n=10 for the adenoma group, n=10 for the stage I–II group, n=9 for the stage III–IV group). *P<0.05 vs. normal group; ^ P<0.05 vs. adenoma group; # P<0.05 vs. stage I–II cancer group. CD, cluster of differentiation.

Article Snippet: The tissue slides were blocked for 10 min at room temperature (25°C), washed with phosphate-buffered saline (PBS), then incubated with the following anti-human antibodies at room temperature (25°C) for 2 h in moist dark chambers: Monoclonal mouse IgG1 against CD3 (#sc-20047), polyclonal rabbit IgG against CD4 (#sc-7219) and CD8 (#sc-7188; all from Santa Cruz Biotechnology, Inc., Dallas, TX, USA; dilution, 1:100); monoclonal mouse IgG2a against CD45RO (#ab86080; dilution, 1:10), monoclonal rabbit IgG against CEACAM6 (#ab134074; dilution, 1:400) and monoclonal mouse IgG3 against FOXP3 (#ab450; 1:50 dilution; all from Abcam, Cambridge, MA, USA).

Techniques: Expressing